</p>
<p>
The UltraView system is a spinning disc confocal, providing
optical sectioning while still permitting rapid imaging, on the
order of 20 frames/s. It has 6 laser lines, 405, 488, 514, 532,
568 and 633. This allows a very wide range of fluorescent probes
to be used on the system. The instrument is also fitted with a
photo-bleach/activation unit, which can be used to provide either
single spots or arbitrary shapes via line or raster scans. This
instrument is particularly useful for doing live cell imaging as
the high imaging speed and low background provide crisp images of
even fast moving objects in live cells.
</p>
<?php
$nasmyth->draw_description();
?>
</div>
<div id="rightpanel">
<img src="../images/sdisk_thumb.jpg" width="200" />
<ul>
<li><a href="http://micronwiki.bioch.ox.ac.uk/wiki/UltraView_spinning_disk_(Nasmyth)">Spinning Disc Wiki</a></li>
<li><a href="http://www.perkinelmer.com/pages/020/cellularimaging/technologies/spinningdiskconfocal.xhtml">Perkin Elmer</a></li>
<li><a href="../resources/Nasmyth Ultraview spinning disk.pdf" target="_blank">Specifications</a></li>
<li><a href="../resources/Ultraview Vox User Protocol Nasmyth 01.pdf" target="_blank">Spinning Disc Users Manual</a></li>
</ul>
</div>
</div>
<?php
sheets from opposite sides perpendicular to the detection objective.
This vastly reduces light exposure and therefore bleaching of the
sample and enables penetration depths of up to 100 µm. It is possible
to image in single or multiview mode, the latter through rotating
the sample and imaging stacks at six to eight different angles,
which can then be recombined into a 360° view. Specimens are
embedded in 1% low-melting agarose or similar inside glass
capillaries and then extruded and imaged in buffer.
</p>
<p>
This system is fitted with an incubation chamber with temperature
and CO2 control.
</p>
<?php
$z1->draw_description();
?>
</div>
<div id="rightpanel">
<img src="../images/lightsheet_thumb.jpg" width="200" />
<ul>
<li><a href="../resources/lightsheetusersguide.pdf">Users Guide</a></li>
</ul>
</div>
</div>
<?php
include "../footer.html";
?>
<p>
Fast Live mode: In this mode it is able to grab simultaneous
images in multiple colours, extremely rapidly and with very
accurate timing control.
</p>
<p>
3D Structured Illumination mode: in this mode the system uses
structured illumination and computer processing to generate 3D
image stacks with twice the conventional resolution, ie just
over 100nm in XY and about 250 nm in Z.
</p>
<?php
$omxv3->draw_description();
?>
<h4>Drawer configurations</h3>
<p>
The OMX V3 BLAZE comes with 2 drawers with the following
configurations:
</p>
<p>
<img src="../images/V3_standard_drawer.jpg" width="500"
alt="OMX V3 standard drawer" title="OMX V3 standard drawer"/>
</p>
<p>
<img src="../images/V3_live_drawer.jpg" width="500"
<p>
Fast Live mode: In this mode it is able to grab simultaneous
images in multiple colours, extremely rapidly and with very
accurate timing control.
</p>
<p>
3D Structured Illumination mode: in this mode the system uses
structured illumination and computer processing to generate 3D
image stacks with twice the conventional resolution, ie just
over 100nm in XY and about 250 nm in Z.
</p>
<?php
$omxv2->draw_description();
?>
</div>
<div id="rightpanel">
<img src="../images/OMXV2_thumb.jpg" width="200" />
<br>
<ul>
<li><a href="http://www.cshlpress.com/pdf/sample/LiveCell2.pdf" title="OMX book chapter">OMX book Chapter</a></li>
<li><a href="http://msg.ucsf.edu/sedat/omx/" title="Sedat Lab OMX">Sedat Lab OMX</a></li>
<li><a href="http://www.api.com/deltavision-omx.asp" title="Applied Precision">Applied Precision</a></li>
<li><a href="http://www.OMXwiki.com" title="OMX wiki">OMX Wiki</a></li>
<li><a href="http://micronwiki.bioch.ox.ac.uk/wiki/OMX_V2" title="OMX V2 Wiki">Micron OMX V2 Wiki</a></li>
<li><a href="../resources/immunostaining_protocol_SIM.pdf" title="Immunostaining Protocol for SIM">Immunostaining Protocol for SIM</a></li>
</ul>
</div>
<?php
include "../scripts/microscopes.php";
use MicronOxford\OpticalMicroscope;
use MicronOxford\SolidStateLaser;
use MicronOxford\GasLaser;
use MicronOxford\Objective;
$zippy = new OpticalMicroscope("zippy", "API DeltaVision Core", "New Biochemistry Room 00-031", array(), array(new Objective(60, 1.35, "Oil UPlanSApo")));
?>
<?php
$zippy->draw_header();
?>
<?php
$zippy->draw_description();
?>
</div>
<div id="rightpanel">
<img src="../images/DVcore_thumb.jpg" width="200" />
<ul>
<li><a href="http://www.api.com/deltavision-omx.asp" title="Applied Precision">Applied Precision</a></li>
</ul>
</div>
</div>
<?php
include "../footer.html";
?>
</div>
<div id="mainbody">
<div id="leftpanel">
<?php
include "../scripts/microscopes.php";
use MicronOxford\OpticalMicroscope;
use MicronOxford\Objective;
$bungle = new OpticalMicroscope("bungle", "API DeltaVision Elite", "New Biochemistry Room 00-031", array(), array(new Objective(60, 1.35, "Oil UPlanSApo")));
?>
<?php
$bungle->draw_header();
?>
<?php
$bungle->draw_description();
?>
</div>
<div id="rightpanel">
<img src="../images/DVcore_thumb.jpg" width="200" />
<ul>
<li><a href="http://www.api.com/deltavision-omx.asp" title="Applied Precision">Applied Precision</a></li>
</ul>
</div>
</div>
<?php
include "../footer.html";
?>
</div>
<p>
The UltraView system is a spinning disc confocal, providing
optical sectioning while still permitting rapid imaging, on the
order of 20 frames/s. It has 6 laser lines, 405, 488, 514, 532,
568 and 633. This allows a very wide range of fluorescent probes
to be used on the system. The instrument is also fitted with a
photo-bleach/activation unit, which can be used to provide either
single spots or arbitrary shapes via line or raster scans. This
instrument is particularly useful for doing live cell imaging as
the high imaging speed and low background provide crisp images of
even fast moving objects in live cells.
</p>
<?php
$barr->draw_description();
?>
</div>
<div id="rightpanel">
<img src="../images/sdisk_thumb.jpg" width="200" />
<ul>
<li><a href="../resources/Barr_Ultraview_Users_Manual.pdf">Users Manual</a></li>
<li><a href="http://micronwiki.bioch.ox.ac.uk/wiki/UltraView_spinning_disk_(Barr)">Spinning Disc Wiki</a></li>
<li><a href="http://www.perkinelmer.com/pages/020/cellularimaging/technologies/spinningdiskconfocal.xhtml">Perkin Elmer</a></li>
<li><a href="../resources/Nasmyth Ultraview spinning disk.pdf">Specifications</a></li>
</ul>
</div>
</div>
<?php
use MicronOxford\Laser;
use MicronOxford\Objective;
$FV1000 = new OpticalMicroscope("FV1000", "Olympus Fluoview FV1000", "Dunn School of Pathology Room 214.00.25", array(new SolidStateLaser(405), new SolidStateLaser(488), new Laser(488, "Pulsed"), new GasLaser(488, "Argon"), new GasLaser(515, "Argon"), new GasLaser(568, "Argon"), new GasLaser(633, "Argon")), array(new Objective(10, 0.4, "UPlanSApo (air)"), new Objective(20, 0.75, "UPlanSApo (air)"), new Objective(40, 1.3, "Oil UPlan FLN"), new Objective(60, 1.35, "Oil UPlanSApo"), new Objective(100, 1.4, "Oil UPlanSApo")));
?>
<?php
$FV1000->draw_header();
?>
<p>
This microscope is an inverted IX81 motorized microscope equipped
with FV1000 Point scanning laser and Becker and Hickel FLIM system
</p>
<?php
$FV1000->draw_description();
?>
</div>
<div id="rightpanel">
<img src="../images/FV1000_thumb.jpg" width="200" />
<ul>
<li><a href="../resources/Olympususersguide.pdf" >Users Guide</a></li>
<li><a href="../resources/FV1000.pdf">Specifications</a></li>
<li><a href="http://www.becker-hickl.de/">Becker and Hickel</a></li>
<li><a href="http://micronwiki.bioch.ox.ac.uk/wiki/Olympus_FV1000_Confocal">Olympus Wiki</a></li>
</ul>
</div>
use MicronOxford\Objective;
$FV1200 = new OpticalMicroscope("FV1200", "Olympus Fluoview FV1200", "Dunn School of Pathology Room 214.00.25", array(new SolidStateLaser(355), new SolidStateLaser(405), new SolidStateLaser(488), new GasLaser(488, "Argon"), new GasLaser(515, "Argon"), new GasLaser(559, "Argon"), new GasLaser(635, "Argon")), array(new Objective(10, 0.4, "UPlanSApo (air)"), new Objective(20, 0.75, "UPlanSApo (air)"), new Objective(40, 1.3, "Oil UPlan FLN"), new Objective(60, 1.4, "Oil UPlanSApo"), new Objective(100, 1.4, "Oil UPlanSApo")));
?>
<?php
$FV1200->draw_header();
?>
<p>
This microscope is an inverted IX83 motorized microscope equipped
with Sensitvie GASP detectors, a 355nm DNA damage laser and an
environmental chamber for live cell work.
</p>
<?php
$FV1200->draw_description();
?>
</div>
<div id="rightpanel">
<img src="../images/FV1200_thumb.jpg" width="200" />
</div>
</div>
<?php
include "../footer.html";
?>
</div>
</body>
conditions up to 1 µm into the cell. Both methods are used to
localise single molecules beyond the diffraction limit. TIRF and
near-TIRF localisation is limited to 2D with no z-information.
Localisation accuracy depends on a high number of detected photons
and on only a fraction of molecules being in a fluorescent state at
any one time. The latter is achieved in PALM by the activation of
only a few fluorophores at the same time, which then bleach
irreversibly, and in dSTORM by reversible switching between a
fluorescent on-state and a dark off-state. PALM uses photoactivatable
fluorescent proteins and dSTORM images fluorescent organic dyes.
Cells need to be in an aqueous medium and for dSTORM need to be
imaged in switching buffer.
</p>
<?php
$palm_tirf->draw_description();
?>
</div>
<div id="rightpanel">
<img src="../images/PALM_thumb.jpg" width="200" />
<ul>
<li><a href="../resources/PALM_Microscope_Users_Manual.pdf">Users Manual</a></li>
<li><a href="../resources/PALM-TIRF_Users_Protocol.pdf">Users Protocol</a></li>
</ul>
</div>
</div>
<?php
include "../footer.html";
